Takami Sho, Li Yu and colleagues (Tsinghua University) unveil migratory autolysosome disposal, a response to lysosomal damage where cells expel LAMP1-LC3 positive structures via autolysosome exocytosis, requiring au
Takami Sho, Li Yu and colleagues (Tsinghua University) unveil migratory autolysosome disposal, a response to lysosomal damage where cells expel LAMP1-LC3 positive structures via autolysosome exocytosis, requiring autophagy machinery, SNARE proteins, and cell migration. This novel mechanism highlights the intricate relationship between cell migration, organelle quality control, and extracellular vesicle release. https://hubs.la/Q02RwkPy0
Berrak Ugur, Pietro De Camilli (Yale Medicine) and colleagues characterize the bridge-like lipid transfer protein VPS13B and report its localization between Golgi cisternae and its impact on Golgi complex reformation
Berrak Ugur, Pietro De Camilli (Yale Medicine) and colleagues characterize the bridge-like lipid transfer protein VPS13B and report its localization between Golgi cisternae and its impact on Golgi complex reformation after its BFA-induced dispersion. They also provide evidence for its functional partnership with FAM177A1, a newly identified Golgi complex protein. https://hubs.la/Q02RmkyD0
Noah de Leeuw, Rashmi Budhathoki, Liam Russell, Dinah Loerke, and Todd Blankenship (University of Denver) examine the mechanical limitations that nuclear volumes impose on epithelial remodeling in Drosophila. They identi
Noah de Leeuw, Rashmi Budhathoki, Liam Russell, Dinah Loerke, and Todd Blankenship (University of Denver) examine the mechanical limitations that nuclear volumes impose on epithelial remodeling in Drosophila. They identify two primary mechanisms—nuclear dispersion and nuclear deformation—that permit the resolution of internuclear tensions. Failures in both pathways lead to epithelial extrusion. https://hubs.la/Q02RdLPB0
Mechanisms leading to abnormal distribution of neural progenitors during cortical development in the context of subcortical heterotopia associated with EML1 mutations remain unknown. Using a forebrain-specific mouse model and mutant human cells, Donia Za
Mechanisms leading to abnormal distribution of neural progenitors during cortical development in the context of subcortical heterotopia associated with EML1 mutations remain unknown. Using a forebrain-specific mouse model and mutant human cells, Donia Zaidi, Fiona Francis and colleagues (Inserm, Sorbonne University Paris) innovatively demonstrate that by restoring microtubule function, abnormal progenitor distribution and heterotopic volume are significantly reduced. https://hubs.la/Q02QZygh0
New from Madison Smith, Lincoln Gay, and Markus Babst (School of Biological Sciences, University of Utah): The rapid swelling of yeast during hypoosmotic conditions is supported by the transport of the membrane and
New from Madison Smith, Lincoln Gay, and Markus Babst (School of Biological Sciences, University of Utah): The rapid swelling of yeast during hypoosmotic conditions is supported by the transport of the membrane and proteins from the ER to the cell surface. This transport seems to be mediated by the fusion of the cortical ER with the plasma membrane at membrane contact sites. https://hubs.la/Q02QBL1w0
Zachary Wilson, Adam Hughes and colleagues (The University of Utah) use electron tomography and time-lapse fluorescence microscopy to observe that mitochondrial-derived compartments (MDCs) are generated from outer mitocho
Zachary Wilson, Adam Hughes and colleagues (The University of Utah) use electron tomography and time-lapse fluorescence microscopy to observe that mitochondrial-derived compartments (MDCs) are generated from outer mitochondrial membrane extensions that repeatedly elongate, coalesce, and invaginate to secure membrane cargo and cytosol within a distinct, protected domain. https://hubs.la/Q02L7nV70
See a related study from the Hughes lab: https://hubs.la/Q02L71F-0
Cells need to grow at the right time, and Marcus A. Harrell, Ziyi Liu, Maitreyi Das and colleagues (Boston College) show that endocytosis helps reset the growth switch (Cdc42) by clearing out the growth-inhibiting protei
Cells need to grow at the right time, and Marcus A. Harrell, Ziyi Liu, Maitreyi Das and colleagues (Boston College) show that endocytosis helps reset the growth switch (Cdc42) by clearing out the growth-inhibiting protein (Pak1). This recycling process keeps growth cycling smoothly. https://hubs.la/Q02GQgDQ0
Human culture cells don’t always divide symmetrically. Alexandre Thomas and Patrick Meraldi (Université de Genève) reveal that centrosome age can lead to unevenly sized spindles. This unexpected asymmetry results in
Human culture cells don’t always divide symmetrically. Alexandre Thomas and Patrick Meraldi (Université de Genève) reveal that centrosome age can lead to unevenly sized spindles. This unexpected asymmetry results in daughter cells of different sizes via kinase Plk1. https://hubs.la/Q02GQ9lx0
Weijing Yao, Yingcong Chen, Yi Zhang, Shu Zhong, Jingjing Tong, Du Feng, Cong Yi (Zhejiang University) and colleagues show that calcium is a critical signal linking environmental sensing to #autophagy initiation by
Weijing Yao, Yingcong Chen, Yi Zhang, Shu Zhong, Jingjing Tong, Du Feng, Cong Yi (Zhejiang University) and colleagues show that calcium is a critical signal linking environmental sensing to #autophagy initiation by triggering Atg11–Bmh1/2–Snf1 complex assembly, which governs Atg1 activation upon glucose starvation. https://hubs.la/Q02F_Flq0
Xiaoting Chao, Yihong Yang, Huaqing Cai and colleagues (Institute of Biophysics, CAS) identify a RasGAP complex that plays a specific role in the regulation of macropinosome formation. Their results demonstrate that both deletion and overexpression of th
Xiaoting Chao, Yihong Yang, Huaqing Cai and colleagues (Institute of Biophysics, CAS) identify a RasGAP complex that plays a specific role in the regulation of macropinosome formation. Their results demonstrate that both deletion and overexpression of this complex compromise macropinocytic activity, highlighting the importance of fine-tuning Ras activity for efficient macropinocytosis. https://hubs.la/Q02CjwSF0
Sophie Gray, Cecile Fort, and Richard John Wheeler (Nuffield Department of Medicine, University of Oxford) use high-frame-rate, dual-color fluorescence microscopy to visualize IFT trafficking in the beating flagella
Sophie Gray, Cecile Fort, and Richard John Wheeler (Nuffield Department of Medicine, University of Oxford) use high-frame-rate, dual-color fluorescence microscopy to visualize IFT trafficking in the beating flagella of Leishmania mexicana, a uniflagellate human parasite. This revealed differing sensitivity of IFT train speed to genetically induced, mechanical, and natural changes in beating. https://hubs.la/Q02zkKyR0
Morgan Pimm, Jessica L. Henty-Ridilla and colleagues (Upstate Medical University) show that IQGAP1 coordinates actin assembly via transient pausing of events and by displacing prominent plus-end binding proteins includin
Morgan Pimm, Jessica L. Henty-Ridilla and colleagues (Upstate Medical University) show that IQGAP1 coordinates actin assembly via transient pausing of events and by displacing prominent plus-end binding proteins including formin (mDia1), capping protein (CP), and mDia1-CP “decision complexes.” https://hubs.la/Q02yp6jZ0
Jason Casler, Laura Lackner and colleagues (Northwestern University) reveal that the ER-localized protein Scs2 interacts with a FFAT motif in the C-terminus of the mitochondria–PM tether Num1 to form a tripartite mitoch
Jason Casler, Laura Lackner and colleagues (Northwestern University) reveal that the ER-localized protein Scs2 interacts with a FFAT motif in the C-terminus of the mitochondria–PM tether Num1 to form a tripartite mitochondria–ER–PM contact site. Loss of the Num1–Scs2 interaction severely perturbs mitochondrial division rates. Unexpectedly, they also identify a novel role of mitochondria–ER–PM contact sites in regulating PM PI(4)P metabolism. https://hubs.la/Q02yhGWK0
Carlo Barnaba, David Broadbent, Jens Schmidt and colleagues (Michigan State University) develop K-FOCUS for high-throughput analysis of autophagy foci in human cells and demonstrate that glucose starvation downregulates autophagy via AMP-activated protein
Carlo Barnaba, David Broadbent, Jens Schmidt and colleagues (Michigan State University) develop K-FOCUS for high-throughput analysis of autophagy foci in human cells and demonstrate that glucose starvation downregulates autophagy via AMP-activated protein kinase, which prevents phagophore tethering to donor membranes to inhibit autophagosome maturation. https://hubs.la/Q02y6vS50
Collectively invading cells and single cells co-exist within a heterogeneous tumor. Sung Bo Yoon, Adam I. Marcus and colleagues (Emory University School of Medicine) show that collectively invading cells abundantly s
Collectively invading cells and single cells co-exist within a heterogeneous tumor. Sung Bo Yoon, Adam I. Marcus and colleagues (Emory University School of Medicine) show that collectively invading cells abundantly secrete laminin-332, which bolsters the invasion of single cells via Rac1 activation through integrin α6/β4 binding. This finding suggests a novel commensal interaction between invasive distinct subpopulations. https://hubs.la/Q02r8YF-0
