The queen is unmarked, but still pretty obvious because her workers are giving her away in this clip.
We mark queens with a small dob of paint, these days from a paint pen. Non-beeks assume that this is to help finding her, and it might help the novice, but like most long timers I spot queens about as quickly either marked or unmarked - ones instincts are just honed to all sorts of clues.
These clues included knowing where in the comb she will most likely be about her business, how the bees behave differently as you get closer to her working through the comb, knowing the kind of places she hides if she is alarmed, and - as in this clip - the attendant bees highlighting her with this circling behaviour.
A relaxed queen that you've not alarmed is much more likely to be easy to spot. If you do alarm her she starts to run around to hide from you and things become much trickier. She will even hide under the workers making the paint mark useless for locating her no matter how garish the colour you pick.
This all give more reasons if you needed them to work smooth and gentle: everything, including finding your queen, is easier if the whole hive is relaxed. I am both pretty much immune to bee stings now and also work in high quality bee suits, the aggression of the bees toward me isn't an issue if provoked, it is factors like this just making every job slower if you trigger their defensive instincts that makes me prize hives with a good temperament.
Aside from the queen running away a defensive hive will also run around everywhere making it much harder to look into the comb to assess diseases in the larva, and also cover all the contact areas where the hive goes back together and so get squashed - emiting pheromones as they die further provoking the hive and it my experience meaning the hive will be more alert to interference for a few weeks after a bad day. In the most extreme cases I've experienced angry bees cover the mesh hood in front of my face making it com
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