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Amir Hamza
2024.02.28
15th graduate students conference, poster presentation.
Enjoying Chuseok Holydays...
429 Coffee Shop
[KakaoMap] ę°ė ėļė˛ė ė ëļė ė§ë´ëĻŦ 429-13
https://kko.to/ju7nkKuDT_
ChIP-PCR experimental approaches (Day-1/ part-2)
1. Continue sonication total in 10 times.
2. In between, freshly prepared TES buffer.
3. Pre-booked A/G beads were washed 2X.
4. ChIP wash buffer with appropriate protease and phosphatase inhibitors.
5. Sonication samples were centrifuged at 13,000 RPM.
6. Then, checked chromatin distribution by running 1% agarose gel electrophoresis.
7. Additional 5X sonication done, in total 15X.
8. Chromatin were then pre-planning for 4 h at 4°, 5 rotation per minute.
9. Take out supernatent and appropriate amounts (1ug Ab for 100ug chr) of antibody were added for IP overnight.
Day1 completed... soon will upload Day2
Day1/ Part2 coming soon....
#vegitables #plantation #yeard #reels
ChIP-PCR experimental approaches (Day-1/ part-1)
1. Freshly prepared 5%BSA in 1X PBS
2. Protein A/G beads blocking by 5% BSA
3. Take out samples from -80°C
4. Prepare ChIP lysis buffer with appropriate protease and phosphatase inhibitors.
5. Resuspended crosslinked samples by lysis buffer and kept for 10 min.
6.In between a 1% agarose gel prepared to see the chromatin distribution.
7. At 15X each 20 pulses each samples sonication for 10 times
Day1/ Part2 coming soon....
#research #experiment #ChIP #PCR #reels
After 24 hours stimulation by LPS, cells were harvesting with crossing-linking by 1% Formaldehyde and stopping by 0.125 M glycine. Let's see the entire experimental approaches...
#reels #viral #GS25 #no_shopkeeper #buy_yourself
I was preparing LPS stimulated cancer cell (A549) as well as ctrl set for ChIP PCR.
#wetlab
#research
#experiment
#reels #concert #korea Amir bin Ali
Making gel... for SDS-PAGE
My first drone shot.
It's crushed during first takeoff.
N.B: Toy drone factđđ
Visited Incheon, South Korea on 2023.01.21